Frequently Asked Questions

Quick answers about ordering, shipping, storing and growing your liquid culture. Still stuck? Email us.

Ordering

No. We sell all of our mushroom liquid cultures through this website.

Each syringe is a 10 ml luer-lock syringe of liquid culture, which is living mushroom mycelium in a sterile nutrient broth. It ships with a sterile needle and an alcohol prep pad.

Shipping

We ship to all 50 states and Washington, D.C. We do not ship to PO boxes, to APO, FPO, or DPO addresses, or outside the United States.

Hawaii requires an extra step. Hawaii law treats fungi, including live mushroom cultures, as microorganisms, and the Hawaii Department of Agriculture must approve an import application (Form PQ-7) before any live culture enters the state. We ship to Hawaii like any other state. You are responsible for getting that approval from the department's Plant Quarantine Branch before your order arrives.

We ship orders within 1 to 2 business days. Shipping is a flat $14.99 to the contiguous United States and Washington, D.C., and a flat $32.99 to Alaska and Hawaii.

Yes. After you place an order, you get a confirmation email saying we received it and are processing it. When your order ships, you get a second email with a tracking number and an estimated delivery date.

Yes. Liquid culture is alive, and long exposure to heat or freezing cold can kill it. During extreme heat or cold, we may hold your order for a few days and will email you if we do. Bring the package inside soon after it arrives and store the syringes as described under storage below.

First, contact the carrier with your tracking number to see if they can help. Then contact us. We decide these case by case and may offer a discounted price or a coupon code to reorder.

Storage

Keep them in the refrigerator, at about 36 to 46°F (2 to 8°C), in the dark. Do not freeze them, because ice kills the mycelium. Leave the cap on until you use the syringe.

Pink oyster is the exception. It is a tropical species and cold kills it, so keep pink oyster syringes at room temperature, about 65 to 75°F (18 to 24°C), out of direct sunlight, and never in the refrigerator.

Use refrigerated cultures within 3 months of delivery and pink oyster within 1 month. The mycelium stays alive longer than that, but it grows more slowly as it ages, so a fresh culture colonizes grain fastest. Each product page gives the storage and use-by time for that species.

Growing
  1. Work in a still air box or in front of a laminar flow hood. Wipe your work surface, gloves, and the outside of your grain jars or bags with 70% isopropyl alcohol.
  2. Let the syringe warm to room temperature, then shake it for a few seconds to break up the mycelium.
  3. Check the broth. It should be clear, with white to cream clumps of mycelium. See the contamination questions below if it is not.
  4. Attach the needle without touching the luer-lock tip or the needle. Wipe each injection port with the alcohol prep pad.
  5. Inject 1 to 3 ml through the injection port of each quart jar of sterilized grain, or 2.5 to 5 ml into each grain bag. One syringe covers 3 to 10 jars.
  6. If you reuse the syringe later, heat the needle in a flame until it glows red and let it cool first. Recap the syringe and store it as before.
  7. Keep the jars or bags at room temperature, out of direct sunlight. Oyster jars usually show white growth within a week. Slower species, such as reishi and maitake, take longer.

No. Liquid culture goes straight into sterilized grain, which is the main reason growers use it. Agar is an optional check. Put a drop from the syringe on an agar plate, and in 3 to 5 days you will see whether it grows clean white mycelium or shows contamination, before you commit a large batch of grain.

A liquid culture is living mushroom mycelium growing in a sterile nutrient broth. Each of ours starts from one isolated culture, so every jar you inoculate grows the same mycelium. The mycelium is already growing, so it starts colonizing grain within days.

A spore syringe holds dormant spores in sterile water. Spores have to sprout and pair up before they form mycelium, so they start slower, and each one is a new genetic mix, so results vary from jar to jar. Most growers who buy spores grow them out on agar first to pick a vigorous, clean culture. A liquid culture skips that step, which is why we sell liquid cultures.

Oyster mushrooms. They colonize fast, tolerate small mistakes, and grow on pasteurized straw, hardwood sawdust, or used coffee grounds. Lion's mane is a good second species. Wine cap is the easiest choice for growing outdoors in a garden bed of wood chips or straw.

Shiitake, maitake, enoki, nameko, and reishi take longer to colonize or need more control over temperature, so try them after a successful oyster grow. Enoki and nameko fruit best in cool conditions.

If you grow golden oyster, keep it indoors and bag spent blocks before you throw them out. Golden oyster has escaped from home grows into forests in several states, and Michigan's state agencies have warned growers about it.

Liquid culture does not go straight into bulk substrate. Inject it into sterilized grain first, then mix that colonized grain, called grain spawn, into a pasteurized or sterilized substrate.

  • Oyster mushrooms grow on pasteurized straw, hardwood sawdust, used coffee grounds, or a 50/50 mix of hardwood sawdust and soy hulls.
  • Lion's mane, chestnut, pioppino, maitake, enoki, and nameko grow best on hardwood sawdust supplemented with bran or soy hulls.
  • Shiitake and reishi grow on supplemented hardwood sawdust or on hardwood logs.
  • Wine cap grows outdoors in beds of hardwood chips or straw.

Yes. Send your question through our contact form. Our support hours are Monday to Friday, 9 to 5 Mountain Time.

Contamination

Let the syringe stand for an hour. A clean culture has clear broth with white to cream wisps or clumps of mycelium that settle to the bottom. The clumps are the culture and are normal. Shake the syringe to break them up before use. Broth that stays cloudy or milky after it settles, a slimy film, green, black, or other colored growth, or a sour smell means the culture is contaminated. Contact us within 30 days of delivery with a photo and we will replace it.

We replace a culture that arrives contaminated, or that shows no growth within 14 days after you inject it into properly sterilized grain. If every jar from one syringe grows the same mold or bacteria, send us photos and we will look at it. Other contamination that shows up after you inoculate grain, agar, or substrate depends on your sterile technique, equipment, and growing space, so we cannot cover it. Our Refund and Return Policy has the details.

Safety and legality

No. The syringe holds mycelium in a nutrient broth for starting cultures. Do not eat, drink, or inject it. Eat only the mushrooms you grow, cooked, and only once you are sure they are the species you planted. Outdoor beds can sprout wild mushrooms, and some wild mushrooms are poisonous.

Returns

No. Liquid cultures are a live product, so we cannot accept returns. We do replace cultures that arrive damaged, contaminated, or as the wrong variety, and cultures that show no growth on properly sterilized grain within 14 days. See our Refund and Return Policy for how to ask.

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Grow Guide

Every stage from syringe to harvest, with a species table and fixes for common problems.

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